A Protein Sensory System Comprising a Fluorescent Conjugated Polyelectrolyte and DNA Aptamer 


Vol. 52,  No. 6, pp. 373-378, Dec.  2015
10.12772/TSE.2015.52.373


PDF
  Abstract

A water-soluble anionic conjugated polyelectrolyte was successfully synthesized via the Suzuki cross-coupling reaction in the presence of a palladium catalyst. Using this fluorescent polyelectrolyte, a new concept for a rapid, label-free lysozyme-sensing method is proposed via possible naked-eye detection of the emission color change. Intermolecular exciton migration in the conjugated polyelectrolyte-based complex was adopted to enhance the selectivity and sensitivity for lysozyme sensing by the formation and dissociation of the polymer-lysozyme assay complex in the absence and presence of the anti-lysozyme aptamer, respectively. The polymer-lysozyme complex showed red emission because of cooperative aggregation of the conjugated polyelectrolyte and lysozyme. Upon exposure to the aptamer, the complex dissociated into individual molecules, resulting in a transparent blue-emitting solution. This occurred because lysozyme is released from the complex by the aptamer via the more favorable binding between the two molecules (lysozyme and aptamer).

  Statistics
Cumulative Counts from November, 2022
Multiple requests among the same browser session are counted as one view. If you mouse over a chart, the values of data points will be shown.


Search
(IN TITLE, AUTHOR, ABSTRACT,KEYWORDS)

Advanced Search ▼

POPULAR KEYWORDS
(TOP 10 KEYWORDS)

Recent Publications
(LAST 3 YEARS)

pISSN : 1225 - 1089
eISSN : 2288 - 6419

한국연구재단 우수등재학술지 유지 (2022년)