Physico-chemical Properties and In vitro Cellular Response of Electrospun Polyurethane Nanofibers Enriched with Copper Chloride and Natural Fish Head Powder
Vol. 23, No. 11, pp. 3022-3027,
Nov. 2022
10.1007/s12221-022-0404-z
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Abstract
Tissue engineering strategies include successful management of bone injuries with biomaterials to develop bonelike
structures. The production of natural biomaterials for this purpose helps both to ensure tissue integrity and to present
alternative products for clinical purposes. However, natural materials are mostly used together with synthetic or metallic
materials due to their low stability and mechanical properties for bone tissue regeneration. There is a clinical need for
material combinations that have both antimicrobial properties and the ability to increase osteogenic induction. This work
aims to fabricate a nanofiber scaffold using polyurethane (PU) loaded with natural fish head powder (FHP) from
Argyrosomus regius species and copper (II) chloride (CuCl2) using the electrospinning technique in order to respond to this
clinical need. There has not been any material combination in the literature that contains these compounds, supports bone
differentiation of stem cells, and prevents bacterial adhesion. To evaluate our composite materials morphology, chemical
structure, wettability, and thermal analysis were studied with different techniques. Anti-adhesive properties of the
Streptococcus mitis (S. mitis) on composite materials were tested with bacterial colonization, and osteogenic inductive
properties by human bone marrow mesenchymal stem cells (hBMSCs) with and without osteogenic differentiation medium
were performed with cell viability assay, Alizarin Red staining, Ca deposition and SEM analysis. According to FESEM-EDS
and ATR_FTIR data, the additives were highly incorporated into the PU nanofibers. The produced materials had randomly
oriented nanofiber with a porosity of ~72 %. The FHP loaded nanofiber had an average diameter of ~185 nm. The swelling
behavior of FHP-loaded PU roughly decreased for 3, 5, and 7 days due to the hydrophobic character of the sample. An
increasing percentage of FHP decreased the bacterial colonization of S. mitis. PUn-CuCl2-FHP nanofibers did not exhibit any
cytotoxic effect on the hBMSCs. According to Alizarin Red Staining and Ca deposition analysis, stimulation with both the
material and the osteogenic medium triggered the bone differentiation of the hBMSCs in a combined manner at days-14.
Based on the results, it can be deduced that natural fish head is a promising additive material for fabricating bone-like
biomaterials.
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Cite this article
[IEEE Style]
Bet, "Physico-chemical Properties and In vitro Cellular Response of Electrospun Polyurethane Nanofibers Enriched with Copper Chloride and Natural Fish Head Powder," Fibers and Polymers, vol. 23, no. 11, pp. 3022-3027, 2022. DOI: 10.1007/s12221-022-0404-z.
[ACM Style]
Bet. 2022. Physico-chemical Properties and In vitro Cellular Response of Electrospun Polyurethane Nanofibers Enriched with Copper Chloride and Natural Fish Head Powder. Fibers and Polymers, 23, 11, (2022), 3022-3027. DOI: 10.1007/s12221-022-0404-z.