Microstructure, Mechanical, and In Vitro Performance of a Novel Combination of Layered Double Hydroxides and Polycaprolactone Electrospun/3D Printed Scaffolds for Bone Tissue Engineering 


Vol. 24,  No. 9, pp. 3085-3099, Sep.  2023
10.1007/s12221-023-00310-9


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  Abstract

Currently, material synthesis and processing developments allow the design of increasingly advanced scaffolds for bone tissue engineering. The purpose of this study is to fabricate hybrid scaffolds by embedding electrospun polycaprolactone (PCL) or layered double hydroxides (LDH)/PCL nanofiber mats into 3D printed circular PCL grids with 400 µm strands using a PCL solution as glue. Structural analysis revealed that LDH increased surface roughness in PCL mats in addition to reducing fiber diameter. FESEM images showed that the size of the 3D printed strands and pores was about the same as in the original design, and nanofiber mats were flawlessly placed between the 3D printed grids. The porosity of the scaffolds was determined through BET analysis. Young’s modulus of the scaffolds was determined using a compressive test conducted in dry and wet conditions. Hybrid scaffolds with LDH/PCL nanofiber mats showed significantly higher Young’s modulus than 3D printed grids and hybrid scaffolds with PCL nanofiber mats (P < 0.05). In vitro studies showed the positive effect of LDH on enhancing hybrid scaffolds’ bioactivity and biodegradation rate. The calcium/phosphate ratio in the hybrid scaffold containing LDH was closer to the stoichiometric calcium/phosphate ratio in natural bone. MG-63 cell lines were cultured to assess the scaffold’s biocompatibility. Cell adhesion, alkaline phosphatase activity, and calcium deposition were significantly enhanced in hybrid scaffolds with LDH/PCL nanofiber mats. Considering the results, this hybrid scaffold exhibits favorable bone tissue engineering characteristics.

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  Cite this article

[IEEE Style]

G. Belgheisi, M. H. Nazarpak, M. Solati‐Hashjin, "Microstructure, Mechanical, and In Vitro Performance of a Novel Combination of Layered Double Hydroxides and Polycaprolactone Electrospun/3D Printed Scaffolds for Bone Tissue Engineering," Fibers and Polymers, vol. 24, no. 9, pp. 3085-3099, 2023. DOI: 10.1007/s12221-023-00310-9.

[ACM Style]

Ghazal Belgheisi, Masoumeh Haghbin Nazarpak, and Mehran Solati‐Hashjin. 2023. Microstructure, Mechanical, and In Vitro Performance of a Novel Combination of Layered Double Hydroxides and Polycaprolactone Electrospun/3D Printed Scaffolds for Bone Tissue Engineering. Fibers and Polymers, 24, 9, (2023), 3085-3099. DOI: 10.1007/s12221-023-00310-9.

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